berg_8e_testbank_chapter05[1] - Chapter 5 Exploring Genes and Genomes Multiple-Choice Questions 1 split(s DNA into specific fragments A B C D E Plasmids

berg_8e_testbank_chapter05[1] - Chapter 5 Exploring Genes...

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Chapter 5 Exploring Genes and GenomesMultiple-Choice Questions1)_____ split(s) DNA into specific fragments.A)PlasmidsB)Reverse transcriptaseC)Polymerase chain reactionD)Dideoxy nucleotide based sequencingE)Restriction enzymesAnswer: ESection: 5.12)A pattern of DNA fragments can serve as a _____ of a particular DNA molecule.Section: 5.13)The most common type of DNA sequencing is _____.Section: 5.1
4)Current DNA sequencing commonly uses _____ base analogues.Section: 5.15)What was the first free-living organism to have its genome sequenced? A)Homo sapiensB)Escherichia coliC)Drosophila melanogasterD)Haemophilus influenzaeE)Canis domesticusAnswer: DSection: 5.36)Who developed the polymerase chain reaction?Section: 5.17)A plasmid is an example of a common ______.
Section: 5.28)pUC plasmids are useful for screening cells that contain recombinant DNA because they contain the _____ gene.Section: 5.29)What enzyme is used to create DNA from RNA?A)RNA polymeraseB)DNA polymeraseC)reverse transcriptaseD)RNA–DNA transcriptaseE)None of the answers is correct.Answer: CSection: 5.210)What type of vector is used to produce large amounts of a desired protein in a bacterial cell?
ESection: 5.2Fill-in-the-Blank Questions11. The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is __________________.Section: 5.212. ___________________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.Section: 5.113. Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as ___________________.Section: 5.214. The Sanger technique for sequencing DNA involves the use of __________________ nucleotide analogs that terminate chain elongation.Section: 5.115. In solid-phase synthesis of oligonucleotides, the 2'deoxyribonucleotide-3'-phosphoramidite is added to the _______ end of the growing oligonucleotide.Section: 5.116. PCR is the abbreviation for ____________________________, which is an in-vitro technique used to make multiple copies of a DNA molecule.Section: 5.117. Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium.Section: 5.218. The enzyme _________________ can be used to add nucleotides to the 3' end of DNA.Section: 5.219. A gene’s function can be studied by inactivating the gene by a process known as gene disruption or _______________.Section: 5.3
20. cDNA attached to a microscope slide forms a _________________ used to study gene expression levels.Section: 5.4Multiple-Choice Questions21) The biological role of restriction enzymes in bacteria is to A)repair DNA. B)induce DNA crossover. C)cleave foreign DNA. D)join gaps in DNA.E)None of the answers is correct.Ans: C Section: 5.1

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