Bio 101 Lab Writeup

Bio 101 Lab Writeup - Christopher Aydin Section X4 Thursday...

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Christopher Aydin Section X4 Thursday 2:15-5:15 Effects of Inhibitors, Enzyme Levels, and Substrate Levels on the rate of enzyme activity Abstract Enzyme activity can be affected by many different things. Experiments will be done to see the rate at which the enzyme can react. The experiments include see the rate of enzyme activity at different enzyme concentrations or substrate concentrations. Also, experiments will be done to see the rate of the enzyme in different pH level or temperatures. All of the things done to the enzyme played a role on the rate at which the enzyme reacted. Enzymes have optimum points at where the react at the fastest rate. Introduction The points to these experiments are to see what has an effect on an enzyme. Each assay works by different variables added to a constant. An experiment can have the same amount of substrate, but have a different amount of enzyme or vise versa. We used sodium molybdate, sodium phosphate, and phenyl phosphonate to see if these substances could inhibit the enzyme. An inhibitor is a substance that decreases the rate of or stops completely a chemical reaction (“Dictionary” Online). The enzyme used in these experiments will be alkaline phosphate. The substrate the enzyme will be reacting upon will be pNPP (Davis and Martin, 2006). Materials and Methods Different amounts of buffer, pH 8, and substrate, pNPP, with sodium molybdate, inorganic phosphate, and phenyl phosphate were assayed with 1.0mL of enzyme, alkaline phosphate, to determine if the amount of buffer had an effect on the enzyme activity (Davis and Martin, 2006). Enzyme concentration, alkaline phosphate, was assayed by diluting the enzyme with water. The same amount of buffer, pH 8, and substrate, pNPP, was added to see if the
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enzyme concentration had an effect on the rate of the enzyme activity (Davis and Martin, 2006). Different amounts of substrate, pNPP, was assayed with the same amounts of buffer, pH 8, and
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Bio 101 Lab Writeup - Christopher Aydin Section X4 Thursday...

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