EC.2 - TA: Edward Chen [email protected] Section: Mondays...

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BIBC 102: Metabolic Biochemistry (Prof. Scheffler) TA: Edward Chen [email protected] Section: Mondays 4-5pm Centr 218 OH: Wednesdays 10-11am @ CLICS Handout #2 A. Example of coupling an endergonic reaction with an exergonic reaction: 1. Glutamate + Ammonia Î glutamine Δ G1 > 0 2. ATP + H2O Î ADP + Pi Δ G2 < 0 3. ATP + glutamate + ammonia Î glutamine + ADP + Pi o Δ G3 = Δ G1 + Δ G2 < 0 B. Oxidation-Reduction Reactions (LEO GER //// OIL RIG) 1. reduced = 2. oxidized = RT [electron acceptor] eq C. E = Δ Eo’ - ----- ln ------------------------- nF [electron donor] eq Δ G’ o = -nFE’ o (Relation between Δ G and Δ E) D. Example of Redox Reaction: 1) pyruvate + 2e- + 2H+ Î lactate E’ o = - 0.185 volts 2) NAD+ + 2e- + H+ Î NADH E’ o = - 0.320 volts 3) NADH Î NAD+ + 2e- + H+ E’ o = E. Glycolysis! (pg. 524) Location: Two phases: Net Products: Phosphofructokinase-1 Aldolase Reaction
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BIBC 102: Metabolic Biochemistry (Prof. Scheffler)
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Unformatted text preview: TA: Edward Chen [email protected] Section: Mondays 4-5pm Centr 218 OH: Wednesdays 10-11am @ CLICS F. Example Problem (NAD+): 1) Give the structural formula of NAD+; also show the reduced form with only the “business end” of the molecule. 2) Illustrate the use of this cofactor with the final reaction in glycolysis catalyzed by lactate dehydrongenase (structural formulae required for the two small carbon compounds). 3) The rate of such reaction is to be measured, using a spectrophotometer. The progress of reaction can be followed. Explain how this can be done. 4) If [1-14C] pyruvate is given to a yeast extract, would you expect to be able to obtain radioactive ethanol? Explain your answer with the help of structural formulae. G. Basic Sugars and their roles in Glycolysis: o Fructose = o Galactose = o Mannose = o Glycogen = o Sucrose = o Lactose =...
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This note was uploaded on 05/24/2008 for the course BIBC 102 taught by Professor Price during the Winter '02 term at UCSD.

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EC.2 - TA: Edward Chen [email protected] Section: Mondays...

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