EC.3 - BIBC 102 Metabolic Biochemistry(Prof Scheffler TA...

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BIBC 102: Metabolic Biochemistry (Prof. Scheffler) TA: Edward Chen [email protected] Section: Mondays 4-5pm Center 218 OH: Wednesdays 10-11am @ CLICS Images taken from Principles of Biochemistry , 4 th edition,by Lehninger et al, 2005 Handout #3 (2/6/06) A. UDP-glucose Structure: How to get from UDP-galactose to UDP-glucose: B. Pathways Catabolic o Glycogenolysis: o Glycolysis: Anabolic o Gluconeogenesis: o Glycogenesis: C. Glycogenolysis: 3 major enzymes: o glycogen phosphorylase o glycogen debranching enzyme o Phosphoglucomutase Reducing end and non-reducing end See slide 7-21, bottom half D. Glycogen phosphorylase Sequential removal of non-reducing end of glucose from glycogen and turns them into glucose 1-phosphate. (Glu) B n B + Pi (Glu) B n-1 B + Glu 1-P cofactor: E. Glycogen debranching enzyme Basically, debranches glycogen 2 steps: o Transferase activity At, (2, 4), cuts off branch and adds it to the end to form (1,7) o Glucosidase activity Breaks off last branched glucose and frees it, leaving a linear polymer
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BIBC 102: Metabolic Biochemistry (Prof. Scheffler)
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