Lab Report 3- Bacteriological Examination of Water, Presumptive and Confirmed Tests of Water Sample

Lab Report 3- Bacteriological Examination of Water, Presumptive and Confirmed Tests of Water Sample

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Lab Report 3: Bacteriological Examination of Water, Presumptive and Confirmed Tests of Water Sample III Madison Epting Section 12 November 13, 2016 This report represents my individual effort. I did not receive or offer aid to anyone when performing this assignment, nor did I plagiarize any material. Signed: __________________________________________________________
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Introduction Significance of bacteriological water testing Before public health standards and the strict regulations from the government, most infectious diseases were spread through contaminated water. A man by the name of John Snow was able to prove that the cholera epidemic in London during the 1840s, was a result from sewage flow into the Thames River from a nearby hospital that housed cholera patients. Cholera is an intestinal disease than can cause death within hours after the first symptoms of vomiting or diarrhea [1]. The water from Thames River was used as drinking water and therefore the disease entered the bodies of multiple well people and was spread all throughout nearby cities through human to human contact. In this time period, people did not have running water or toilets in their houses so they used communal pumps to do just about anything that involved water. With this being known, Snow tracked down multiple answers to his theory, his solution was for the pump handle to be taken off. Doing this slowed the contamination of cholera to a stop and proved that water borne illnesses exist [1]. Biological water testing is important because of how many fatal diseases can live in water. Most water borne illnesses are formed because of fecal contamination, or not properly cleansing or filtering water that will be consumed. Microbiological water analysis relies mainly on a fecal indicator. This indicator looks for strains of Escherichia coli, Vibrio cholera, Salmonella enteric, Shigella dysenteries and other emerging pathogens [2]. Wastewater discharge into fresh and coastal waters are the major source of these fecal microorganisms reaching humans [2]. With the use of bacteriological water testing, contaminated water can be stopped before being bottled and sent to a consumer, also stopping the spread of water borne illnesses. Use of Coliforms in Water Quality Testing
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Coliforms are apart of the family Enterobacteriaceae. These bacteria make up about 10% of the intestinal microorganisms of humans and other animals and are a use of indicator organism. Coliforms are defined as facultative anaerobic, gram-negative, nonsporing, rod-shaped bacteria that ferment lactose with gas formation. Three tests that use coliforms are the presumptive, confirmed and completed test [4]. Brief Description of the Presumptive Test The presumptive test is used in water quality testing to see if the water contains any lactose-fermenting bacteria. This can be determined after incubating the inoculated water sample and it contains bubbles in any of the lactose broths. This would be a positive test for fermentation, and the test would “presumed” to contain coliforms. The presumptive test is also
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  • Fall '13
  • Rudolph
  • Bacteria, Escherichia coli, water sample

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