Syllabus Lab (Fall 2016)

Syllabus Lab (Fall 2016) - MICR 3051 LAB SYLLABUS FALL 2016...

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MICR 3051 LAB SYLLABUS FALL 2016 LAB MANUAL: Custom manual (available at the Clemson University Bookstore) LAB ROOM: 128 Life Sciences Building (all sections except 13) 136 Life Sciences Building (section 13 only) OBSERVATION ROOM: 132 Life Sciences Building TA Name TA Email TA Phone TA Office/Lab TA Assistant Name STUDENT LEARNING OUTCOMES/GOALS 1 : 1. Students will be able to conduct and understand various microbiological lab procedures. [B, C, H] 2. Students will be able to collect, analyze, and present scientific data. [B, C, E, H] 3. Students will be able to write scientific lab reports. [B, C, E, H] 1 Letters in brackets refer to general education competencies (Clemson University Undergraduate Announcements 2016 – 2017, p. 36) TENTATIVE LABORATORY SCHEDULE Week Page 1. Aug. 22 – 25 Brightfield Microscopy 7 Aseptic Technique 17 Smear Preparation 27 Simple Staining 31 Ubiquity of Bacteria 33 Lab Safety Activity handout 2. Aug. 29 – Sept. 1 Gram-Staining 37 Pure Culture Techniques 41 Cultural Characteristics 53
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Week Page 3. Sept. 5 – 8 Spore Staining (Schaeffer-Fulton) 57 Lab Report 1 Due Acid-Fast Staining 61 Negative Staining 67 Motility Determination 69 TBA 4. Sept. 12 – 15 Oxidation/Fermentation 75 General Unknown 87 Unknown Project (continues for several weeks) 5. Sept. 19 – 22 Bacterial Growth Curve 107 Hydrolytic/Degradative 97 Triple Sugar Iron Agar 103 Effects of Oxygen on Growth 119 pH and Microbial Growth 127 Water Activity & Osmotic Pressure 131 Unknown Project (make slants) 6. Sept. 26 – 29 MID-TERM LABORATORY EXAM Unknown Project (testing) 7. Oct. 3 – 6 Ultraviolet Light: Lethal Effects 135 Evaluation of Antiseptics 139 Antimicrobic Sensitivity Testing 145 Evaluation of Alcohol 155 Alcohol Fermentation 179 Unknown Project (complete) 8. Oct. 10 – 13 Alcohol Fermentation Observation 155 Reductase Test 159 Enumeration of Bacteria 163 Bacterial Counts of Foods 175 Nitrogen Cycle: Ammonification 183 Food Safety Worksheet TBA 9. Oct. 17 – 20 Complete Ammonification 183
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Unknown Report Due Bacteriological Examination of Water 189 (Lab Report 2) (Presumptive Test) Bacteriophage Titer 203 Set up for Replica Plating 209 TBA Week Page 10. Oct. 24 – 27 Bacteriological Examination of Water 189 (Confirmed Test) Bacterial Mutation 213 Mutant Isolation by Replica Plating 209 The Staphylococci 217 ( Culturing for S. aureus, Part 1) student notes TBA 11. Oct. 31 – Nov. 3 Transformation 227 DNA Extraction 237 Effects of Lysozyme on Bacterial Cells 241 Culturing for S. aureus (Part 2) student notes TBA Nov. 7 – 10 FALL BREAK (NO LABS!) 12. Nov. 14 – 17 A Synthetic Epidemic 247 Lab Report 3 Due Slide Agglutination Test 255 (Culturing for S. aureus, Part 3) student notes Morbidity and Mortality Weekly Report 261 TBA Nov. 21 – 24 THANKSGIVING BREAK (NO LABS!) 13. Nov. 28 – Dec. 1 FINAL LABORATORY EXAM ATTENDANCE: Attendance at laboratory sessions is mandatory ! Regular and punctual attendance at all laboratory sessions is a student obligation, and each student is personally responsible for completing all of the assigned procedures. The labs are scheduled for three hours , so plan accordingly. If a student leaves before being dismissed by the laboratory instructor, it will count as an unexcused absence.
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