Micro 305 Lab 1 - Welcome to Micro 305 Lab Contact Info...

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Welcome to Micro 305 Lab! Contact Info: Carly Dameron Office: 159 LSF (office hours by appointment) Email: [email protected]
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Agenda for Today Go over syllabus Lab Safety procedures Sign the sheet! Lab tour and lab coats Lab 1 Aseptic Technique Smear Preparation Simple Staining Brightfield Microscopy Ubiquity of Bacteria Clean Up
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Syllabus Attendance is mandatory! Contact me within 48 hours of absence Required Materials Lab manual Loose-leaf binder & student notes Long pants, closed-toed shoes, hair tie Protective eyewear
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Syllabus - Grading Lab is 25% of your MICR 3050 grade Pre-lab Quizzes – 10% Every week at beginning of lab Notebook – 5% Lab Reports 1 and 3 – 20% Unknown Project/Report – 35% Midterm Lab Exam – 15% Final Lab Exam – 15%
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Lab coats, long pants, closed-toed shoes Bring safety goggles No phones or laptops! Proper waste disposal NOTHING goes down the sink! Tie back long hair Safety shower and eyewash Lab Safety
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Aseptic Technique – pg. 19-23 Important!! 1. Disinfect your bench 2. Always work close to your flame 3. Flame your loop before removing bacteria 4. BRIEFLY flame the mouth of the tube before removing bacteria 5. BRIEFLY flame the mouth of the tube before capping 6. Flame your loop after you have inoculated broth or agar slant/plate
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Smear Preparation – pg. 28 To get a good staining image, you have to start with a good smear.
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