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Unformatted text preview: Because the bacterial chromosome is transferred to F- bacteria in a linear order, and because the rate of transfer is constant, the order and approximate distance between bacterial genes can be estimated using interrupted mating experiments. HFr a + b + e + g + h + str s x F- a- b- e- g- h- str r Mix two strains together and break apart at various times after mixing and plate on medium containing streptomycin. Score the str r colonies for the presence of a + b + , e + , g + and h + . Markers nearer the origin will transfer first and to higher frequency. Time between the first appearance of the markers is the distance separating them in minutes. Partial diploids (merodiploids) allow complementation tests in normally haploid bacteria! MORE LATER!! a + b + BIO 320: Lecture 12 Bacterial genetics Conjugation: a natural transfer of genes between bacteria. F+ (donor cell) F- (recipient) 1) F (fertility) factors are capable of transferring themselves from F + donor cells to F- recipients....
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