CHP 9 DNA Based Information Technologies (4pp)

CHP 9 DNA Based Information Technologies (4pp) - Chapter 9...

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1 Chapter 9 1 Chapter 9 Homework Assignment • We will not cover the entire chapter. Please use the lecture notes and the Review Sheet for testable material • I have decided to alter the homework assignment for Chapter 9. The following problems will be due once we finish the chapter: 3, 4, 7, 8, 10 Chapter 9 DNA-Based Information Technologies Chapter 9 3 DNA-Based Information Of all the natural systems, living matter is the one which, in the face of great transformations, preserves inscribed in its organization the largest amount of its own past history. - Emile Zuckerkandl and Linus Pauling -omics …is the study of…: Genomics : the full complement of an organism's genes. Proteomics : the full complement of an organism's proteins: Transcriptomics : an organism's RNA transcribed from its DNA Metabonomics : an organism's metabolite profiles Structural genomics : the 3-D structures of an organism's proteins and RNAs Pharmacogenomics : the interaction between genes and gene products and medications. How an individual's genetic inheritance affects the body's response to drugs. Chapter 9 4 DNA Cloning: The Basics • Understanding of any complex problem usually begins by breaking that problem into smaller, less complex units. • This method is also used by the biochemist who isloates and studies the individual components of a biological system, its DNA, RNA, proteins, etc. • However, the sheer amount of DNA found in living cells makes the process very challenging. • Think about trying to find a very small needle in a VERY large haystack • Technologies have since been developed that allow us as scientists to locate, isolate, prepare and study small segments of DNA derived from much larger chromosomes. • These techniques of DNA cloning led the way in the development of all of the –omics fields! Very Smart Guys
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2 Chapter 9 5 DNA Cloning: The Basics • DNA Cloning deals with separating a specific gene or DNA segment from a larger chromosome or DNA vehicle, attaching it to a smaller molecule of carrier DNA and then replicating this modified DNA 10 3 or 10 6 times. • Cloning of DNA has five general steps: 1) Cutting the DNA at a precise location 2) Selecting a small molecule of DNA capable of self-replication 3) Joining two DNA fragments covalently 4) Moving this recombinant DNA from the test tube into a host cell 5) Selecting of identifying host cells that contain the recombinant DNA • These methods are collectively called recombinant DNA technologies or genetic engineering Chapter 9 6 DNA Cloning: The Basics Restriction endonucleases are the DNA cutters for genetic engineering who recognize and cut DNA at locations specific to each enzyme • These enzymes were discovered by Werner Arber, who found that they served as an immune response in bacteria (chewed up foreign DNA) • These enzymes recognize specific sequences of DNA called Palindromes • There are three types of enzymes: Type I : cleaves 400 – 7000 bp from the recognition site
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This note was uploaded on 06/03/2008 for the course BIO 150 taught by Professor Kore during the Spring '08 term at Abraham Baldwin Agricultural College.

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CHP 9 DNA Based Information Technologies (4pp) - Chapter 9...

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