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homework 7 - BICD 100 Spring 2008 Page 1 of 3 S Brody ext...

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BICD 100 Page 1 of 3 S. Brody, ext. 42619 Spring 2008 4125 Muir Biology HOMEWORK SET #7 — KEY 30 pts total 1. ( 15 pts total ) The time of entry results indicate the following order: pur + ~16' ~18' ~22' pro + lac + marker locus time of entry origin The second plating, on medium containing proline, lactose and streptomycin, selects for pur + ; thus, the partial diploid may be represented as: pur + pro + lac + ! " # $ pro lac pur Since the selection for pur + requires the stable integration of the pur + locus, the exchange labeled above is required. Exchange or or will then complete the integration of pur + . The genotypes of the colonies on plates A and B and their origins are as follows: Plate A Colony Type Genotype # Origin (Exchanges) a) ( 5 pts ) i.e., unable to grow on B Not seen on plate B Not seen on plate B pur + lac + pro pur + lac pro 9 10 , , Plate B ( 5 pts ) pur + lac + pro + pur + lac pro + 29 1 , , , , b) ( 5 pts ) Map distances region 10 + 1 49 Total = 22.4% 9 + 1 49 = 20.4%
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BICD 100 Page 2 of 3 S. Brody, ext. 42619 Spring 2008
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