Homework 7 Key

Homework 7 Key - BICD 100 Spring 2008 Page 1 of 2 S Brody...

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BICD 100 Page 1 of 2 S. Brody, ext. 42619 Spring 2008 4125 Muir Biology 1 HOMEWORK SET #7 (Covering lectures #22,23) Due Friday, May 30, 2008 ***PLEASE PUT YOUR NAME AND SECTION NUMBER IN INK OR TYPED ON TOP OF YOUR HOMEWORK SET*** 30 pts total (15 pts) 1. In a time of entry experiment, an Hfr lac + pro + pur + str s strain was mixed with an F lac pro pur str r strain and the mixture plated at intervals on a media which allows all streptomycin resistant colonies to grow (i.e., pur + or ; pro + or ; lac + or ). The following results were obtained (after replica plating). 10 20 30 40 50 60 100 80 60 40 20 Time (minutes) Frequency of traits from Hfr parent pro + lac + pur + pro pur lac = proline requirer = purine requirer = lactose non-fermenter (can grow on glucose) At 25 minutes, a sample is plated on minimal medium to which proline, lactose and streptomycin has been added and containing an indicator dye on which colonies that can ferment lactose turn red and those that cannot remain white (A) . When these colonies had formed, they were replica plated
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