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Unformatted text preview: BICD 100 Page 1 of 2 S. Brody, ext. 42619 Spring 2008 4125 Muir Biology 1 HOMEWORK SET #7 (Covering lectures #22,23) Due Friday, May 30, 2008 ***PLEASE PUT YOUR NAME AND SECTION NUMBER IN INK OR TYPED ON TOP OF YOUR HOMEWORK SET*** 30 pts total (15 pts) 1. In a time of entry experiment, an Hfr lac + pro + pur + str s strain was mixed with an F – lac – pro – pur – str r strain and the mixture plated at intervals on a media which allows all streptomycin resistant colonies to grow (i.e., pur + or – ; pro + or – ; lac + or – ). The following results were obtained (after replica plating). 10 20 30 40 50 60 100 80 60 40 20 Time (minutes) Frequency of traits from Hfr parent pro + lac + pur + pro pur lac – – – = proline requirer = purine requirer = lactose non-fermenter (can grow on glucose) At 25 minutes, a sample is plated on minimal medium to which proline, lactose and streptomycin has been added and containing an indicator dye on which colonies that can ferment lactose turn red...
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This note was uploaded on 06/07/2008 for the course BICD 100 taught by Professor Nehring during the Spring '08 term at UCSD.
- Spring '08