BIO 206L: Exercise 2 Analysis
Please answer the following questions during lab. Keep in mind that you need to work independently. Write in
complete sentences, using proper grammar and spelling when an explanation is required. Be complete yet
concise in your answers. Submit your report as a pdf file on Canvas
before
the beginning of the next lab.
1. What is Köhler illumination and why is important?
2. How does the “field of view” change with magnification? Relate this to the rule that one should always use the low-power scanning objective first to locate a specimen. How does the “depth of focus” change as magnification increases? Are thick (e.g., Elodea) or thin (e.g., prepared Paramecium) samples of specimens preferred at high magnifications?
3. How does one improve resolution? What does a numerical aperture (N.A.) indicate? What is the relationship between resolving power and numerical aperture?
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- Spring '08
- Unknown
- Biology, Bone marrow, white blood cell
