Enzyme Lab

Enzyme Lab - Movement of Materials through Cell Membranes...

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Movement of Materials through Cell Membranes 1
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Jenine Hajjar BI 101 Honors General Biology October 26, 2007 2
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Abstract The purpose of this experiment was to analyze the factors affecting enzyme activity. The first part of the experiment demonstrated the activity of an amylase enzyme using an iodine test and the second part of the experiment demonstrated the effect of pH on the amylase activity. The objectives of this lab were also to describe enzyme kinetics and to determine the optimum pH for the digestion of starch by the enzyme amylase. The first part of the experiment demonstrated the digestion of starch by amylase. The end product of digested starch is a sugar called maltose. All together there were six test tubes used in this part. There were three subparts to the test. Each subpart had two test tubes, one containing iodine and one containing Benedict’s solution. The iodine was used to display the activity of the amylase enzyme and the Benedict’s solution was used to reveal the presence of maltose. Part A’s tubes were used as the control. Part B contained unheated enzyme. The remaining two tubes of Part C contained boiled enzyme. The boiling of this enzyme resulted in the denaturing of the enzyme. Part two of this experiment demonstrated the effect of pH on amylase activity. Seven test tubes were filled with solutions of pH 3, 4, 5,6,7,8, and 9, respectively. Starch and enzyme were added to each tube and then tested with for the presence of starch at one minute intervals over a 10 minute period. Part one reveals that starch was digested in test tube 3 and test tube 4 because they differed in color compared to their controls; however tubes 5 and 6 did not differ from their controls because the enzymes were denatured. This shows that amylase will digest starch only if 3
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the starch has been exposed to amylase before it is denatured. Part two reveals that the optimum pH for amylase is 5. This is demonstrated by the quick change in color of the solution. Introduction “Starches are frequently stored by plants and animals for use as an available energy source.” The process of starches being chemically converted into sugar units is called digestion. The sugar unit which this experiment was concerned with was glucose because it is a key compound in cellular metabolism and it contains energy that can be released to do work in the cell. In order for starches to be digested, enzymes, called amylases, must be present. These enzymes will break the starch molecule into smaller and smaller subunits until maltose, which is a reducing sugar, until is obtained. Maltose is then converted into glucose, which is the source of energy for living organisms. Enzymes are globular proteins that act as catalysts for metabolic reactions (Pack 2001). Typically they are organic catalysts which remain unchanged by the chemical reactions in which
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This note was uploaded on 06/09/2008 for the course BIO 101 taught by Professor Borst during the Fall '07 term at Quinnipiac.

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Enzyme Lab - Movement of Materials through Cell Membranes...

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