Photosynthesis Lab

Photosynthesis Lab - Materials and Methods Part 1...

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Materials and Methods Part 1 – Separation of Chloroplast Pigments Part one of this experiment attempted to determine if chlorophyll is one pigment or if it is composed of several different pigments. A spinach leaf was placed on the lower portion of a piece of chromatography paper strop and then the edge of a special wooden tool provided was rolled in a straight line across the leaf. This was perpendicular to the axis of the paper strip. Then this process was repeated about eight times, using different sections of the leaf, until a dark green imprint was obtained on the chromatography paper. After this process was completed, the paper strip was placed into a test tube containing an half inch of benzene-petroleum either. Part 2 – Necessity of Chlorophyll for Photosynthesis Part two of this experiment attempted to determine how it can be shown that chlorophyll is necessary for photosynthesis. A leaf from a variegated Coleus that had been in strong light for several hours was obtained. The leaf was placed in hot water in order to remove the red pigments and then it was placed in a beaker of hot alcohol and heated until color was removed. After the color was removed from the leaf, the leaf was removed from the alcohol and placed in a petri dish containing iodine. The leaf was left in the dish for several minutes. Part 3 – Necessity of Light for Photosynthesis Exercise three of this lab experimentally demonstrated that light is necessary for photosynthesis. A geranium plant was placed in the dark for approximately 48 hours. At the end of the 48 hours, the plants were removed from the dark and black light shields were attached to
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some of the leaves. The plants were then allowed to grow in the light for 24 hours. Another set of geranium plants were kept in constant light for the 72 hour period to act as the control. A leaf with a light shield on it was removed from the plant. The shield was then taken off the leaf. The leaf was placed in a beaker of hot alcohol and heated until pigment was removed. The leaf was
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This note was uploaded on 06/09/2008 for the course BIO 101 taught by Professor Borst during the Fall '07 term at Quinnipiac.

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Photosynthesis Lab - Materials and Methods Part 1...

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