Lecture 8 - The Mitochondrial Generation of ATP

Lecture 8 - The Mitochondrial Generation of ATP - Lecture 8...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
1 The Mitochondrial Generation of ATP Biology 1F25 for Biology Non-Majors Lecture 8 Background Reading Textbook, Chapter 3, especially pages 53-55. The People Who Prepared This Lecture Harry Peery Jeff Stuart The cycle is involved in the catabolism of The cycle is involved in the catabolism of carbohydrates, lipids and amino acids. carbohydrates, lipids and amino acids. Intermediates of the cycle are starting points Intermediates of the cycle are starting points for many biosynthetic reactions. for many biosynthetic reactions. Enzymes of the cycle are in the mitochondria of Enzymes of the cycle are in the mitochondria of eukaryotes or in bacteria. eukaryotes or in bacteria.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 Entry of Pyruvate into the Mitochondrion Entry of Pyruvate into the Mitochondrion Pyruvate translocase transports pyruvate into the mitochondria in symport with H + Symport means the simultaneous active transport of two substances – in this case, pyruvate and hydrogen. Pyruvate dehydrogenase complex Pyruvate dehydrogenase (E1) Dihydrolipoyl transacetylase (E2) Dihydrolipoyl dehydrogenase (E3) Three enzyme types in the complex The pyruvate dehydrogenase complex (PDC) is really composed of many enzymes working together You are not responsible for the names of the individual enzymes, but you should know E1, E2, and E3 and the concepts that are in the text on this slide. 3D structure of the PDC complex (a) Core of the complex (24 E 2 chains) (b) Model of the entire complex: 12 E 1 dimers (blue), 6 E 3 dimers (green) surround the core
Background image of page 2
3 Another artist’s rendition of the PDC Dihydrolipoyl Transacetylase (E2) • Cholangiocytes are cells that line the bile ducts within the liver. • In cholangiocyte mitochondria, but nowhere else, the E2 component mutates. • It is unclear where the mutation occurs. • The gene for E2 is one of the mitochondrial genes that has gone to the nucleus of the cell. AMAs are produced specifically against E2 • It ends up in the cell membrane from which location it activates the T-cells to become professional antigen presenting cells (APCs). • APCs stimulate the formation of antibodies. • In this case, antimitochondrial antibodies (AMAs) are formed specifically against the E2 subunit. • The whole cholangiocyte is destroyed. Kaplan MM; Gershwin ME (2005) N Engl J Med Sep 22; Vol. 353 (12), pp. 1261-73. Narrowed biliary ducts (arrows) and collapsed biliary duct (arrowhead) as a result of primary biliary cirrhosis (early stage from liver biopsy) The result: primary biliary cirrhosis
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 Hepatic cirrhosis • Destruction of the liver by cell death and scar tissue formation is called hepatic cirrhosis, or just cirrhosis for short. • The word ‘primary
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 11

Lecture 8 - The Mitochondrial Generation of ATP - Lecture 8...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online