1The Mitochondrial Generation of ATPBiology 1F25for Biology Non-MajorsLecture 8Background Reading•Textbook, Chapter 3, especially pages 53-55. The People Who Prepared This LectureHarry PeeryJeff Stuart•The cycle is involved in the catabolism of The cycle is involved in the catabolism of carbohydrates, lipids and amino acids.carbohydrates, lipids and amino acids.•Intermediates of the cycle are starting points Intermediates of the cycle are starting points for many biosynthetic reactions. for many biosynthetic reactions. •Enzymes of the cycle are in the mitochondria of Enzymes of the cycle are in the mitochondria of eukaryotes or in bacteria.eukaryotes or in bacteria.
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2Entry of Pyruvate into the MitochondrionEntry of Pyruvate into the Mitochondrion•Pyruvate translocase transports pyruvate into the mitochondria in symport with H+Symport means the simultaneous active transport of two substances – in this case, pyruvate and hydrogen.Pyruvate dehydrogenase complexPyruvate dehydrogenase (E1)Dihydrolipoyltransacetylase (E2)Dihydrolipoyldehydrogenase (E3)Three enzyme types in the complexThe pyruvate dehydrogenase complex (PDC) is really composed of many enzymes working togetherYou are not responsible for the names of the individual enzymes, but you should know E1, E2, and E3 and the concepts that are in the text on this slide.3D structure of the PDC complex•(a) Core of the complex (24 E2chains)•(b) Model of the entire complex: 12 E1dimers (blue), 6 E3dimers (green) surround the core