Lecture 31 - Tumour Growth Factors

Lecture 31 - Tumour Growth Factors - Lecture 31 Background...

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1 Tumour Growth Factors Biology 1F25 for Biology Non-Majors Lecture 31 Background Reading Textbook, Chapter 19. The People Who Prepared This Lecture Harry Peery Alan Castle Last lecture: Kiyono et al.: Cell immortalization occurred when Rb was inactivated and telomerase was active. p53 activity had no effect on mortality/immortality.
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2 Nature 400: 464-468. 1999. Approach: First: transfect normal cells with the Large-T gene. Then, transfect with: (a) no other gene (b) ras oncogene (c) active hTERT gene (d) ras oncogene and active hTERT gene Test for immortality and ability to cause tumours. Results First: Cell Growth and Immortality Cell Growth/ Immortality Symbols: + hTERT; +hTERT, +ras; no other gene; +ras
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3 Hahn et al. Conclusions #1 Cell immortalization occurred when Rb and p53 were inactivated (transfection by Large-T) and telomerase was active (transfection by hTERT). ras oncogene had no effect on mortality/immortality. Results Second: Tumour Formation (each type of cell was injected into mice) Tumour Formation Hahn et al. Conclusions #2 Tumours were formed only when Rb and p53 were inactivated (transfection by Large-T ); telomerase was active (transfection by hTERT ); and when the cell had a ras oncogene.
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4 Hahn et al. Overall Conclusions A minimum of four mutations was necessary for the conversion of a normal cell into a cancer cell. These mutations are: (1) proto-oncogene to oncogene (2) inactivation of one allele in a tumour suppressor gene (3) a mutation in the second copy of the same tumour suppressor gene as in (2) (4) activation of the telomerase gene. Comparison to real life:
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Lecture 31 - Tumour Growth Factors - Lecture 31 Background...

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