Biology Results and Discussions

Biology Results and Discussions - Stephen Jabaut Biology...

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Stephen Jabaut March 26, 2008 Biology Results and Discussions Section 65 Results These different parts of this experiment involved determining the rate of reaction between an enzyme, B-galactosidase, and O-nitrophynal-B-D-galactoside ONPG. The rate of this reaction can be measured by measuring the absorbance of light wavelength of a byproduct of the two components. This byproduct is called O-nitrophenol and can be measured at a wavelength absorbance of 420 nm. ONPG degrades slowly on its own as well, so even when an enzyme reaction does not occur, there could still be trace amounts of O-nitrophenol present, giving off an absorbance reading. Experiment A: This phase of the experiment involved determining the rate of the reaction of different concentrations of enzymes every minute over the course of five minutes. The purpose of this phase was to find a concentration of enzyme that had the most ideal linear curve, and learn about how time effects an enzyme’s reaction. Absorbance readings at 420nm: Group Enzyme Control 1 min 2 min 3 min 4 min 5 min 1 .1 ml .025 .062 .039 .071 .101 .106 2 .2 ml .018 .163 .144 .096 .516 .203 3 .3 ml .044 .050 .145 .151 .163 .228 4 .5 ml .040 .386 .646 .788 1.060 1.150 Experiment B:
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In this phase of the experiment, a .3 mL concentration of the enzyme was heated at 90 degrees Celsius for 5 minutes and then measured for absorbance after another 5 minutes of reaction time. The purpose of this experiment was to learn the effect of high temperatures on an enzyme. Absorbance Readings at 420nm: Group Temperature Time Absorbance 1 90 degrees 5 min .034 2 90 degrees 5 min .008 3 90 degrees 5 min .063 4 90 degrees 5 min .056 Experiment C: In this experiment, Uninduced Enzyme was similarly implemented and allowed to react for five minutes under two different concentrations of enzyme. The purpose of this phase was
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to discover the difference in an induced enzyme vs. an Uninduced enzyme, and to find out the different characteristics of the two. Absorbance readings at 420 nm:
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Biology Results and Discussions - Stephen Jabaut Biology...

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