Mat and Meth - Stephen Jabaut Biology Lab Materials and...

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Stephen Jabaut February 17 th 2007 Biology Lab Alyssa Nielubowicz Materials and Methodologies Onion Root Cells Conditions The dried layer and first outer fleshy layer of bulb scales were removed from the onion and then the onion was submerged the bottom third of the onion in water, leaving it suspended with toothpicks. After two days of soaking, root cells necessary for the experiment exposed themselves and could be removed from the bulb. Roots were cut about 1.5 inches from the tip for the purposes of the experiment. Onion Root Composition The root-cap goes from 0 to .5 mm from the tip. The meristematic region goes from .5 to 1.5mm from the tip, the zone of elongation goes from 1.5 to 3.5mm from the tip, and the zone of maturation extends beyond that. It was noted that these regions contain different cells that are in different phases of mitosis. Because that this is true, it makes the root portion of the onion very desirable for the purposes of the experiment. Chemicals Used on Root Cells A root was soaked in Peroxidase (freshly prepared hydrogen peroxide, chloronapthol, and Tris buffer) to indicate cells in the root with high Peroxidase activity. High Peroxidase activity gave an indication of cells that were dividing. Another root was soaked in 1mg/mL Tetrazolium to
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