lab 6 q2 - do not swell or shrink the cell(Bio 05LA Lab...

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Question 2: How will the isotonic concentrations for RBC’s change with exposure to different solutions of increasing osmolarity but equal concentration? Hypothesis: The isotonic concentrations for RBC’s will decrease with exposure to different solutions of increasing osmolarity but equal concentration. Supporting Facts: 1. The unit of concentration use for aqueous solutions is molarity. Molarity is the number of moles of solute per liter of solution. (Campbell “Biology” 7 th ed. Text,pp.53). 2. Osmolarity is the total solute concentration per liter of solution. (Campbell “Biology” 7 th ed. Text,pp.923). 3. Ionic bonds are formed by a negatively charged ion (anion) and positively charged ion (cation). Ionic compounds are formed by ionic bonds and dissotiate in water. (Campbell “Biology” 7 th ed. Text,pp.41). 4. Isotonic concentration is the molar concentration where the suspending cells
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Unformatted text preview: do not swell or shrink the cell. (Bio 05LA Lab Manual-Lab#6, UCR, Winter ’08 ed., pp2 parag#1). 5. In optical density, the amount of light scattered is proportional to the number of particles in suspension. (Bio 05LA Lab Manual-Lab#3, UCR, Winter ’08 ed., pp3 parag#1). Experimental Strategy: The spectrophotometer will be used to keep track of the presence of intact RBC’s. The number of cells will affect the optical density. The cells that are not osmostically lysed in a certain solution are capable of scattering visible light and therefore that solution will have a high optical density. Prediction: If the isotonic concentrations for RBC’s will decrease with exposure to different solutions of increasing osmolarity but equal concentration, then the molarity of the ionic compounds will be less than the molarity of glucose....
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