DNA replication

DNA replication - DNA replication Critical Enzymes...

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DNA replication Critical Enzymes necessary for DNA replication o DNA polymerases - enzymes responsible for aligning complimentary nucleotides along template strand of DNA Polymerases can only add nucleotides to the 3’ end of the existing DNA chain Polymerases are also responsible for replacing RNA primers with DNA nucleotides Nucleotides added are initially triphosphate monomers and it is the cleaving of two phosphates (as pyrophosphate) by polymerase that allow nucleotides to add to growing DNA strand o Primase - Add a short RNA primer to DNA template strand before polymerases can act. (Primers usually 5-10 nucleotides long) o DNA ligase - joins okazaki fragments together to form complete DNA strand o Topoisomerases - catalyze and guide the unknotting of DNA. Replication - Takes place in a semiconservative manner; after replication each DNA strand will have one new stand and one parental strand. o
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DNA replication - DNA replication Critical Enzymes...

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