micro lab review - Organisms EXERCISE 1 - BRIGHTFIELD...

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Unformatted text preview: Organisms EXERCISE 1 - BRIGHTFIELD MICROSCOPY mixture of purple rods and cocci and pink spirals Spirillum flagella-precipitated dye used-at 1000X cells are maroon and flagella are black-arrangements are monotrichous, polar, amphitrichous, lopho- trichous, and peritrichous Klebsiella bacterial capsule polysaccharide layer outside cell wall most dyes wont adhere so use complex stain (positively stained pink then background is negative stained with dye that doesnt penetrate capsule look for halo (cells and background are pink) Bacillus and Clostridium bacterial endospores heat resistant difficult to stain b/c dehydrated (water-soluble dyes usually used) vegetative cells are pink and spores are green EXERCISE 2 - MICROBIO LAB TECHNIQUES Saccaromyces cerevisiae (yeast/eukaryote) in broth used for wet mount with drop of iodine streaked sabouraud dextrose agar slant (30 degrees) Escherichia coli in broth smear with methylene blue NA plate (37 degrees) Bacillus subtillus on agar slant smear Mix of Bacillus subtilis and Saccaromyces cerevisiae in broth smear 3) GRAM STAIN Escherichia coli on NA plate pink rods Bacillus subtilis on NA slant gram positive rods with clear areas (spores) Mycobacterium smegmatis on TSA slant small thin rods (matchsticks) buried under waxy coating acid fast mix of gram pos (cocci) and gram neg (rods) in broth 5) PURE CULTURE Escherichia coli on TSA plate off white colonies at both room temp and 37 degrees Serratia marcescens on TSA plate (looks very similar to E Coli when gram stained) red at room temp (because of prodigiosin which protects bacteria against UV light) and off-white at 37 degrees pink rods Micrococcus luteus on TSA plate yellow at both temps on TSA plate purple cocci 6 MOTILITY Micrococcus luteus on NB nonmotile Proteus vulgaris on NB motile 7) ANTIBIOTIC TESTING E coli in NB Staphylococcus aureus in NB Proteus vulgaris in NB Pseudomonas aeruginosa in NB 8 STAPH AND STREP Staphylococcus aureus clusters, gram + cocci opaque yellow colonies on plate grow in media with NaCl (selective) catalase positive ferments mannitol, grows quickly (turns MSA yellow) produces alpha toxin that lyses rbc in blood agar medium (clear) beta hemolytic on SBA Staphylococcus epidermidis gram pos cocci, grows slowly, mannitol negative (MSA remains red), nonhemolytic on SBA Streptococcus pyogenes no growth on MSA and beta hemolytic on SBA 9) GRAM NEG ENTERIC PATHOGENS E. coli gram negative rods, lactose +, positive for indole and MR, + for glucose and gas Enterobacter cloacae gram negative rods, lactose +, positive for VP and citrate, + for glucose and gas Proteus vulgaris gram negative rods, lactose -, acid and gas from glucose and sucrose, positive for phenylalanine, urea, and H2S Salmonella enteritidis responsible for intestinal problems gram negative rods, lactose -, acid/gas from glucose, positive for H2S Shigella flexneri...
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micro lab review - Organisms EXERCISE 1 - BRIGHTFIELD...

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