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exam1key - BIO 226R EXAM 1\V)6 Dr Earhart f NAME UT EID 1(5...

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Unformatted text preview: / BIO 226R EXAM 1 \V February 12, 2007 )6/ Dr. Earhart f NAME UT EID 1. (5 points) Name the three most common shapes of bacteria. Include in your answer typical bacterial dimensions. : ,2"! I. Lcyc. (Coccmg‘ 3. (6 points) What are the structural subunits (building blocks) of peptidoglycani S a; ' )- 4 J é‘l “ 2— 1/ 6’ ‘ A D \f / CA anti-L #- mjmm. mo. (3*- , d9 .._ ac; c :h‘ / 1 y I ' ‘H'h‘ma 4 - / ’7» lg; 14. .44 *- WLoe-J . I. r \ f M I .’l 7/ Q’L.A‘_‘._ #J‘H ,0 _ 1’ . ’1 _/ ' / 4. (9 points) Name a unique feature of (i) Archaeal lipids. gamma: gait; [guigd A} Cfltrg in}; c .. - : ? /. (ii) Planctomycetes. Wilda; c: ‘ ( cl‘ 0 Z. 'c“ #— 5 5". 5? big a ’ 3 Flu-*0 air-Il'f'ja‘ I r f .30 (iii) Mycoplasmas #m 452 gggéggég-éq at my Una/C if é'u\ " . f/ a. 1’5 c. A ‘c 3 ’11 €91 If - as. ' f’ ‘ - (11¢ C ‘ (- 5. Question 5 (3 parts) concerns flagella. f” (i) (6 points) Name the three major structural components of flagella. .7“ V gm“. E 1 dine J 4.. 'a 5/ J (ii) (3 points) Where are the flagellar components located in the envelope of a gram- negative bacterium? '1' me 1“ '- é’nhke/ )‘1 Cl)" In it I I l r . ' c: 1 t - J a ‘ C I. .1157! 1 V1 F e ' ca - V Dotch C» W: (iii) (3 points) Where are these components in spirochetes with endoflagella? 3’ 6. Question 6 concerns bacterial growth. (6 points Draw a growth curve for a bacterial culture. Make sure you label the axes. ). (ii) (4 points) Define unbalanced growth and indicate on the above Eowth curve where ,— 9. unbalanced growth is occurring. 7. (3 points) Why are most bacterial experiments conducted with cells in exponential (log) phase? 8. (4 points) Do protoplasts have peptidoglycan? Explain. \ _.-'ll . 1-7-1’ )0}— L/Lm 651+- an-m-L W M M: ' WM 4 ‘1“ cola-4 -4“... 1.41 w .—-' l‘fiflu “‘4‘ . I \ I I I .._.___4.’L... I; I ‘Hu ' 1..-“l.‘.‘.—L ‘ ' I a a A 7 l. Luna... 1 4‘ 5/; [fish la. . ,/'/ 4,, IIW //._pi‘/’;’ 9. (3 points) Are bacteria growing in liquid media in solution or n suspension? Explain your mm a“. - g... .,_i 4. .- -/ _/ - be...“ I I_. ,l ' I Fa 4'33: L n a' 4- (c I “7. 10. (4 points) In the three domain system for classification of life, which domain, if any, does not include microbes? 1' b‘ x. ‘ / I I WW“ "I. "‘ 1 l . (4 points) What is the primary role of peptidoglycan‘? 12. (6 points) Given: The weight of a typical bacterium growing in minimal medium is l picogram (1x lO'IZg). Do you think it would be possible to isolate 1 g of bacteria? Explain how you could do this or why it would not be possible. n. :2. A 13. Question 13 (4 parts, 10 points) concerns the gram stain. (i) How is a gram-stain test performed? /.??L5"\ _hf Ar WM £21.11! "'""- « ‘ ( y . 4 4. I! £4 14. (10 points) Define the following terms. f_2? (iii) Slayers 4 11114; (n ' __... ’4 ~— .n-...'..... l l a...“ 4, ‘ J a w W" 125:4, v H_ ‘1‘ / '6 2" dfiv) PSYChmphile ‘30-'73) (v) Plasmid . 1 {Cu - Asa/414! .._....‘ b”? . ' , l' ' ' ' /-”" I I ' r 15. (5 points) Nam and describe a device to maintain a bacterial culture in balanced growth for long periods (weeks) of time. a f“ 64.3%. "' 16. (4 points) Are viruses microbes? Explain. A.» I g» 1.44sL 1 . I. .14-,1 .741 A..__— N .I: I F "I I1r\‘i"lug'-],l q Jirbulanx, lf (an? Upmf'aw «5-» "Heu- wa I 1?. EXTRA CREDIT (6 points) Given: A bacterial species capable of growth in both a minimal and a rich medium. Describe the gross cellular differences between this species grown in minimal medium and the same species grown in rich medium. [JV-(I “uh ‘“ ‘4, ~ M"M_J_M£LW .4:- L. 4.. '- 1... 4 M' 4-. L I, ' ’ -- .‘J; -’ ' Lug-.- ‘44-‘41." Jun 1M JII' J Rim _r o» rm“: > M ...
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