exam2key - Bio 226R - KEY Exam II March 19, 2007 Dr....

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Bio 226R - KEY Exam II March 19, 2007 Dr. Earhart 1
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1. (8 points) Given the following four types of transport: [p. 100-103] a) Ion-coupled transport b) Facilitated diffusion c) Group translocation (phosphotransferase system) d) ATP binding cassette (ABC) transport Which of these four is found only in prokaryotes? (c) PTS Which requires no energy? (b) Facilitated Diffusion Which is used by E. coli for glucose uptake? (c) PTS Which may require a periplasmic binding protein? (d) ABC transport 2. (2 points) Which of the following inorganic compounds cannot serve as an electron acceptor in anaerobic respiration? [p. 93] a) nitrate (NO 3 - ) b) sulfate (SO 4 2- ) c) monohydrogenphosphate (HPO 4 2- ) d) sulfur (S o ) 3. (2 points) Gluconeogenesis is the synthesis of hexose from one-, two-, three-, or four carbon substrates. [p. 102] 4. (2 points) Anaerobic (strict) bacteria are killed by oxygen. [p. 99] 5. (2 points) Porin proteins form small channels in the outer membrane through which hydrophilic molecules of 600 daltons or less can pass. [p. 99] 6. (15 points) What is the significance of each of these reactions? If oxidation/reduction is involved, indicate the molecules that are oxidized and those that are reduced. [p. 120] (i) 2-oxoglutarate + NH 3 + NADPH + H + Æ glutamate + H 2 0 +NADP + COOH COOH | | CH 2 CH 2 | | CH 2 CH 2 | | C=O H-C-NH 2 | | COOH COOH a) NH 3 assimilation b) Syn of glutamic acid (an amino acid). NADPH 2 is oxidized, oxoglutarate is reduced. 2
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(ii) 3-phosphoglyceraldehyde + NAD + + H 3 PO 4 Æ 1,3 bisphosphoglycerate + NADH + H + [p. 87] H - C = O O | | | H-C-OH C – O - PO 3 H | | H 2 C – O – PO 3 H 2 H-C-OH | H 2 C-O- PO 3 H 2 NAD is reduced 3 PGA is oxidized Importation of P i into organic form Generation of a high energy phosphate (iii) glutamate + phenylpyruvate 2-oxoglutarate + phenylalanine [p. 119] COOH | COOH | | CH 2 | CH 2 CH 2 | CH 2 | | C=O | H-C-NH 2 CH 2 | CH 2 | | COOH | COOH H-C-NH 2 C=O | | COOH COOH Phenylpyruvate is reduced, Glu is oxidized Transamination Syn. of phenylalanine.
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This note was uploaded on 06/16/2008 for the course BIO 226r taught by Professor Latham during the Spring '08 term at University of Texas at Austin.

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exam2key - Bio 226R - KEY Exam II March 19, 2007 Dr....

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