Exam 4A Key - BIO 226R Exam 4A Name UT eID Discussion time...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
BIO 226R Exam 4 A Name:______________________ Discussion time:_______ UT eID:_____________________ 1. You are a graduate student studying growth of E. coli on mannose (a carbohydrate). You identify a transcriptional regulator important for transcription of the mannose utilization genes. Design an experiment to determine if this regulator is a transcriptional activator or repressor. (10 points) Inactivate the regulator genetically. If the mannose utilization genes are constitutively expressed (by examining promoter activity), it is a repressor. If the genes are not expressed it is an activator. A less sensitive way is: inactivate the regulator, if the bacterium now grows on maltose all the time, it is a repressor. If the mutant bacterium doesn’t grow on maltose at all, it is likely an activator. 2. Name three components required for a plasmid to be self-transmissible. (6 points) oriT, tra genes (encoding pilus), mob genes (encoding relaxase, primase, localization proteins) 3. Under the following conditions, will ColE1 plasmid copy number increase or decrease? (2 points each) a. Mutating the plasmid so it doesn’t produce RNAI. ___ increase _________ b. Mutating the plasmid so it doesn’t produce RNAII. ______ decrease _______ c. Mutations increasing the promoter strength of RNAI. ________ decrease_ ____ 4. Define: (3 points each) a. regulon – Set of operons throughout the chromosome that are controlled by the same regulatory protein. b. allosteric effector – Ligand that binds an enzyme at a site not in the active site that changes the conformation (and activity) of the enzyme. c. phenotype – The observable properties of the organism. Ex. Morphology, growth rate,.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
5. You are charged with identifying factors required for alanine biosynthesis in the bacterial pathogen Pseudomonas aeruginosa . You decide to do transposon mutagenesis to identify important genes. P. aeruginosa
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page1 / 5

Exam 4A Key - BIO 226R Exam 4A Name UT eID Discussion time...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online