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Unformatted text preview: Name: 1. Lac Operon A. Given the genotypes, fill in the appropriate phenotypes for LacZ and LacY. The inducer is lactose or IPTG. Phenotypes LacZ expression LacY expression Genotype no inducer inducer no inducer inducer 1 I + O + Z + Y + 2 I S O + Z- Y- 3 I S O C Z + Y + / F’I- O + Z- Y- 4 I + O c Z- Y + / F’ I- O + Z + Y- 5 I- O C Z + Y + / F’ I S O + Z- Y- B. Name one strain that indicates that O C is epistatic to I S , and what data tells you that? Strain 3- Z+ and Y+ data in all four entries Strain 5- Z+ and Y+ data in all four entries. C. A new gene is identified that regulates the Lac operon, called Gene T. An allele Gene T-2 was analyzed. We know that Gene T+ acts in the glucose, ADC1, CRP pathway. The assays of Gene T+ function are done in a I- cell, with glucose present . (The phenotypes would be complicated by reporting LacZ expression with no glucose and with glucose column. However, you can figure this out using this data alone). LacZ expression 1. GeneT+ - 2. GeneT-2 + 3. GeneT-2/ Gene T+ - 4. ADC1* + 5. ADC1* GeneT-2 + 6. ADC1- - 7 . ADC1- Gene T-2 + i. Is Gene T-2 recessive or dominant (circle one) ii. Draw the glucose, Gene T+,ADC1,CRP, LacZ, pathway,ii....
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This note was uploaded on 06/16/2008 for the course ECOL 320 taught by Professor Weinert during the Spring '07 term at University of Arizona- Tucson.
- Spring '07