PS06Sol(hw5)sol07 - Genetics 320. HW # 6 Due Oct11. 1. PCR....

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Genetics 320. HW # 6 Due Oct11. 1. PCR. Start with the genomic DNA and primers shown below. Draw the PCR cycles required to make a doublestranded DNA of 1000 bases (it takes more than 1 cycle!). Darken the sequence where the primers bind each cycle. Label the end of the DNA strands. Label each primer. You need only show how to form ONE piece of doublestranded DNA. B A AGC….TTT AGC….TTT ….GGTCTG ….GGTCTG The primers sequences are identical to the solid lines shown below (A) and above (B). 1000 bases 3’ A 1st cycle done with Primer A, for example. Melt, anneal primer B B 2nd cycle done with primer B. Melt, anneal primer A 3’ 5’ 5’ 3’ 5’ A 3rd cycle done with primer A. Doublestrand DNA of 1000 bases completed. 5 3 template Melt. Use bottom Strand as template Note: You can also have done with starting with the top strand as template Then use Primer B, the Primer A, then primer B.
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2. A mitotic diploid cell is shown. It has the genotype AbBb, or, to show the proper phase, AB/ab. Draw how recombination event(s) in one cell division can generate an ab/ab diploid cell. You can use a simple “X” to mark the position of the crossover(s). Note: Crossovers can occur between 1 and 3, 2 and 4, 1 and 4, and 2 and 3. You must have two crossovers in same cell to get this to work. I’ve shown a G2 cell, and labeled the centromeres for you. Enjoy. A B a 1 2 3 4 Two crossovers as shown. Then co-segregate 1 and 3. 1 3 a b a b This is one of the possible solutions. You could also have the crossovers between 2 and 4, for example. I think those are the only two possibilities.
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3. Describe in a sentence then draw simply what each of the following do: DNA polymerase, RNA polymerase, and a ribosome do. Draw a simple diagram that tells what they do (as we did for our “Gene Stories”!!!). The dots are a lot of codon
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This note was uploaded on 06/16/2008 for the course ECOL 320 taught by Professor Weinert during the Spring '07 term at University of Arizona- Tucson.

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PS06Sol(hw5)sol07 - Genetics 320. HW # 6 Due Oct11. 1. PCR....

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