BIO1140 Chapter 7b - Function of Membranes

BIO1140 Chapter 7b - Function of Membranes - Cellular...

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Cellular Biology Chapter 7 – Membranes: Their structure, function, and chemistry Evidence for the ‘Mosaic’ of proteins Membranes were prepared for electron microscopy using a Freeze fracturing method Membranes were frozen and then split using a diamond knife splitting the lipid bilayer This reveals the inner surface of each membrane The electron microscopes then showed that the proteins are actually suspended within the membranes Integral, Peripheral, and Lipid-Anchored Proteins Integral Proteins Amphiphatic proteins whose hydrophobic regions pass through the hydrophobic layer of the bilayer They also have hydrophilic regions that extend outward from the membrane into the aqueous phase Integral monotopic proteins – embedded in and only protrude from one side of the bilayer Transmembranes – embedded in and protrude from both sides of the membrane (note: examples are for red blood cells) o Can cross the membrane once – singlepass proteins - Hydrophilic C (carboxylic) and N(amino) terminus
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This note was uploaded on 06/17/2008 for the course BIO 1140 taught by Professor Fenwick during the Winter '07 term at University of Ottawa.

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BIO1140 Chapter 7b - Function of Membranes - Cellular...

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