exam 4 Maura answers

exam 4 Maura answers - 1. In the boxes below, write the...

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1. In the boxes below, write the charge that the functional group would possess at pH = 5. Write either +, - or 0 (zero) in the boxes. The nitrogens are in a more acidic environment than their pKa. The oxygens are in a more basic environment than their pKa. 2. In the boxes below, write the charge that the functional group would possess at pH = 12. Write either +, - or 0 (zero) in the boxes. The nitrogens are in a more basic environment than their pKa – but they aren’t able to lose two protons and acquire a negative charge. The oxygens are still in a more basic environment than their pKa. 3. What is the pI of each of these? Asp:____ 2.77 _______ Lys:_________ 9.74 __________ Asn:_______ 5.74 ________ 4. Draw the structure(s) that the three-letter code “Glx” can represent. Glx = Glu + Gln Glu Gln
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5. Draw Arg-Glu-Met. Circle its ionizable groups. Ionizable groups are groups that can lose or gain a proton. 6. If you wanted to separate Asp, Lys and Asn using an electrophoresis gel, at what pH would you run the gel? 5.74 – This is the only pH at which one amino acid will be neutral, one will be positive and one will be negative. Where on the gel would Asp, Lys and Asn be after you run the gel? You can just write “Asp,” “Lys” and “Asn” in the appropriate places. At pH 5.74, Asn is neutral. Asp is at a more basic pH than where it is neutral, so it will be negatively charged, and run toward the positive electrode. Lys is at a more acidic pH than where
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This note was uploaded on 06/19/2008 for the course CHEM 332 taught by Professor Kisslings during the Spring '08 term at Binghamton University.

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exam 4 Maura answers - 1. In the boxes below, write the...

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