DNA_notes - 3 6) 7) Problems: 1) Draw pdGpT 2) Draw pppApC...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
N N N N NH 2 O OH O H H H H P O HO O - NH N N O NH 2 N O OH H H H H O P O O - O O - ApGp Nucleotide polymer notes read 5' to 3' ppdApdC N N N N NH 2 O H O H H H H P O O O - N NH 2 O N O H OH H H H H O P O O - O P - O O O - DNA synthesis in lab starting with nucleosides 1) Protect bases which have amine (ACG) with: A C G N N N H N NH 2 NH N N H N O NH 2 N N H NH 2 O O O O base NH N H O O NH N H O O U T N N N N NH 2 O H OH H H H H HO O O O py N N N N HN O H OH H H H H HO O 2) Protect 5' alcohol with DMTCl Cl OCH 3 CH 3 O O H OH H H H H HO A O DMTCl O H OH H H H H DMTO A O DMTCl= dimethoxytrityl chloride 3) Add phosphorus to 3' via: P Cl N O N O H OH H H H H DMTO A O P Cl N O N O H O H H H H DMTO A O P N O N 4) Add base protected nucleoside and coupling activator tetrazole N N N H N O H O H H H H DMTO A O P N O N O H OH H H H H HO A O + O H O H H H H DMTO A O P O N O H OH H H H H O A O base
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
O H O H H H H DMTO A O P O N O H OH H H H H O A O 5) Oxidize phosphorus with I 2 , H 2 O, py I 2 , H 2 O, py O H O H H H H DMTO A O P O N O H OH H H H H O A O O 6) Deprotect the 5' OH with O CCl 3 OH O H O H H H H DMTO A O P O N O H OH H H H H O A O O O CCl 3 OH O H O H H H H HO A O P O N O H OH H H H H O A O O 7) Deprotect base and phosphorus with NH 3 O H O H H H H HO A P O - O H OH H H H H O A O excess NH
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 3 6) 7) Problems: 1) Draw pdGpT 2) Draw pppApC Solid state synthesis (3' to 5') Prep nucleosides with steps 1-3 O H OH H H H H HO A 1) O O O py 2) DMTCl, base 3) P Cl N O N O H O H H H H DMTO A O P N O N OH O H O H H H H DMTO A O P N O N N N N H N O H O H H H H DMTO A O P O O N O CCl 3 OH 4) 5) I 2 , H 2 O, py O H O H H H H DMTO A O P O O N O 6) O H O H H H H HO A O P O O N O = Repeat 4,5,6 adding a nucleoside each cycle. To exit, add excess NH 3 (deprotect P, base, and bead)...
View Full Document

This note was uploaded on 06/22/2008 for the course CHEM 216 taught by Professor Nolta during the Winter '07 term at University of Michigan.

Page1 / 2

DNA_notes - 3 6) 7) Problems: 1) Draw pdGpT 2) Draw pppApC...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online