HW_1 2007 - BME 339/CHE 339/BIO 335 Fall 2006 Homework #1...

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BME 339/CHE 339/BIO 335 Fall 2006 Homework #1 Assigned: Sept. 6 2007 Due: Thursday, September 13 2007 1) A yeast (CH 1.66 N 0.13 O 0.40 ) is growing aerobic ally on arabinose (C 5 H 10 O 5 ) and ammonium hydroxide (NH 4 OH) with a respiratory quotient of 1.4. a. Determine the yield coefficient , Y X/S , for this system in grams biomass /gram substrate . b. What is the maximum biomass concentration that can be achieved in batch growth for this system if the initial substrate concentration is 10 g/L and the initial biomass concentration is 0.5 g/L. 2) A strain of mold was grown in a batch culture on glucose and the following data were obtained: Time (hr) Cell Concentration (g/l) Glucose conc.(g/l) 0 1.25 100 9 2.45 97 16 5.1 90.4 23 10.5 76.9 30 22 48.1 34 33 20.6 36 37.5 9.38 40 41 0.6 Find: (a) The specific growth rate. (b) The saturation constant Ks (c) The maximum cell concentration that may be obtained if 150 g/l of glucose were used with the same initial inoculum (i.e. cell concentration at t=0).
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HW_1 2007 - BME 339/CHE 339/BIO 335 Fall 2006 Homework #1...

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