DNA-Gel_fromlabTA

DNA-Gel_fromlabTA - DNA fingerprinting and Gel...

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DNA fingerprinting and Gel Electrophoresis Bio200 LAB
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Isolating DNA What you need to do first is isolate DNA from whatever your sample may have been. Blood, hair, semen, or body tissue. Sample Isolated DNA Extraction
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Add Restriction Enzymes After you have obtained DNA from your sample, you add restriction enzymes to the DNA. The restriction enzymes are specialized to recognize certain sequences in the DNA and then cut them accordingly. For example, one might only recognize a sequence only. When it comes across this specific sequence then, it cuts it accordingly forming RFLP’s (Restriction Fragment Length Polymorphisms; which is just a fancy name for the DNA fragments you are left with after the cutting.) TAT ATA TAGCCGA TAT CGAGCCGATTCG ATCGGCT ATA GCTCGGCTAAGC TAGCCGATA TCGAGCCGATTCG ATCGGCTA TAGCTCGGCTAAGC Whole DNA whose sequence is recognized by the restriction enzyme Resulting RFLP’s
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Separating the RFLP’s using gel electrophoresis What you have now after using the restriction
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This note was uploaded on 06/24/2008 for the course BIO 200 taught by Professor Herreid during the Fall '07 term at SUNY Buffalo.

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DNA-Gel_fromlabTA - DNA fingerprinting and Gel...

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