Lecture 38 DNA TECH PT1 slides

Lecture 38 DNA TECH PT1 slides - DNA Technology or Genetic...

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DNA Technology or Genetic Engineering, Part I 6 th ed.: 375-380; 383-385 7 th ed.; 384-389;392-394 406-407 Genetic engineering: manipulation of genes for desired results Other words : -Recombinant DNA technology -Molecular biology -Gene cloning
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Goals : 1.Products - improved crops, livestock - proteins (insulin) 2.Research - Basic knowledge - Medical applications 3.Forensics - “DNA evidence” Gene cloning: making many copies of a desired gene or another piece of DNA
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Bacteria : central role in gene cloning. -Fast-growing -Cheap; high volume -We can take advantage of mechanisms that evolved in bacteria, and use them for our own purposes. Many copies of any gene can be grown in bacteria to - 1. isolate the gene for further study, OR: 2. have bacteria express the gene: isolate the protein
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Our example: express human insulin gene in bacteria; isolate recombinant insulin for treatment of diabetes 1.Isolate DNA from human cells 2.Digest DNA to fragments with a restriction enzyme.
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Restriction enzymes: endonucleases that cleave DNA at specific sequences enzyme
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Most enzymes see palindromes : sequence is same FORWARD on 1 strand and BACKWARD on the other strand AGTACT TCATGA Many restriction enzymes - from different bacteria - Each recognizes a different sequence (4-8 nucleotides)
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This note was uploaded on 06/25/2008 for the course BIO 202 taught by Professor Dean during the Fall '08 term at SUNY Stony Brook.

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Lecture 38 DNA TECH PT1 slides - DNA Technology or Genetic...

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