IontransportationLab4 - Kelly Lunt MCB 32L Nadia Ion...

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Kelly Lunt MCB 32L Nadia Ion Transport by Frog Skin I. Introduction: Background: The common frog is a fresh water inhabitant whose skin is permeable to respiratory gases and water. The frog continually takes up water from the pond and to balance the water intake it excretes hypotonic urine. In that urine NA+ is lost and the frog compensates by taking in NA+ through its skin. The frog’s skin, or epithelial cells, play a major role in maintaining the frog’s internal environment. Hypothesis: The purpose of this lab is to see the effects of different drugs on the frog skin and its ability to transport ions. II. Materials and Methods: Skin from the frog’s belly was dissected and then clamped between two Lucite chambers. Solutions were added to each chamber and were kept oxygenated. The potential difference was then measured using a hand held volt meter. Drugs were also tested on the frog’s skin. Amiloride was added to one chamber and a reading was taken every 15 seconds for the first minute and then every 30 seconds for 2 more minutes. Repeat this step but fill the other side of the chamber with the drug. The drug will only act on one side of
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This note was uploaded on 06/28/2008 for the course MCB 32 taught by Professor Wurmser during the Fall '07 term at University of California, Berkeley.

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IontransportationLab4 - Kelly Lunt MCB 32L Nadia Ion...

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