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sciatic2 - Kelly Lunt October 5 2007 Lab 3 4 Frog Sciatic...

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Kelly Lunt October 5, 2007 Lab 3 & 4 Frog Sciatic Nerve and Action Potential I. Introduction Background : Action Potential is an all or noting occurrence. In running a current through the frog’s sciatic nerve (a bundle of nerve fibers found in the leg) we are able to measure and observe the action potential recorded. However, the experiment only simulates an extracellular response, differing from the computer simulation in which we stimulated an action potential within he axon. The reading represents a summed activity of individual axons and their conducting velocities. Purpose: To determine a compound action potential and different fiber groups using a frog sciatic nerve. To determine the conduction velocity of the fiber component. To establish the refractory period of the nerve. To measure the conduction velocity, we used this equation: x/(t 2 – t 1 ) X: distance the recording electrodes were moved (cm) t 1 : time between stimulus artifact and peak using recording electrodes placed closer. (msec) t 2 : time between stimulus artifact and peak using recording electrodes placed further. (msec) I I.
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