CHEM 239 finalkey-1 - Nothing on this page-or the back of...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Nothing on this page--or the back of any page --will be graded. You may remove this page for scratch paper. Item 1: repeat LEU2-d P6 P5 ura3 (INTRON +) CEN ARS BamHI w + P4 P3 ampR ori repeat P1 P2 EcoRI HindIII Item 2: ___ 6 1 ___ 1 7 ___ 1 7 ___ 2 3 ___ 4 3 ___ 2 4 ___ 3 3 ___ 1 3 ___ 3 7 stillborn d. 3 yrs d. 42 yrs Family One Family Two ___ 5 7 Item 3:
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Final, Genome 371 Name _______________KEY_________________ Fall 2004 Berg/Brewer TA ______________________ 1. Below is a drawing of a transposon constructed by an over-zealous genetic engineer. Help her sort out the elements that are important for a Drosophila P-element from those that are important for a yeast Ty-element. (Also include those components that are important for discovering the site of insertion of the transposon in the genome after transposition.) Some elements are extras. Assume that Reverse Transcriptase and Integrase or Transposase are provided elsewhere. A. Put an X in the box for each feature that is needed. NOTE: The fly and yeast columns were switched on the buff exam. B. Would the yeast CEN interfere with obtaining transposition events in Drosophila? ___No __ Would the yeast CEN interfere with obtaining transposition events in yeast? __Yes ___ Explain these two answers. Yeast centromeres are DNA sequences recognized only by yeast kinetochore proteins. Drosophila kinetochore proteins would not recognize the yeast DNA sequences and would not be affected by their presence. If a yeast transposon contained a CEN, integration would create a chromosome with two centromeres. Microtubule binding in mitosis would tear the chromosome apart. ampR ori w + ARS CEN ura3 (INTRON +) LEU2-d EcoRI HindIII BamHI P1 P2 P3 P4 P5 P6 repeat repeat feature needed P1 P2 repeat ori EcoRI ampR HindIII P3 P4 w+ BamHI ARS CEN ura3 P5 P6 LEU2-d repeat in fly in yeast X X X X X X X X X X X X X X X X X
Background image of page 2
2. Doug Koshland’s plasmid was modified by replacing ARS1 with different ARSs from the yeast genome. When introduced into ade2, ade3, leu2 yeast, they produced the following results on complete plates. A. What phenotypic difference(s) do you note? Cells with the ARS2 plasmid exhibit more sectoring than cells with ARS1 or ARS3, which exhibit similar levels of sectoring. B. What do you conclude from these results? ARS2 may be a less efficient origin of replication than ARS1 or ARS3, causing a higher rate of loss of the plasmid. C. The yeast strain (ade2, ade3, leu2) was mutagenized and point mutations recovered. The pool of mutants was transformed with Doug Koshland’s plasmid. Individual transformants were tested for minichromosome maintenance by looking for sectoring on complete plates. One mutant was discovered that increased white sectoring on complete plates and was identified to be a new mutation in MCM5—a member of a protein complex required for replication initiation. Is this new allele of MCM5 a (circle one) . . .
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 06/29/2008 for the course CHEM 239 taught by Professor Sasaki during the Winter '06 term at University of Washington.

Page1 / 10

CHEM 239 finalkey-1 - Nothing on this page-or the back of...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online