Sample Midterm

Sample Midterm - 1 When twe earhcn atems are ccnnected by a...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 1 . When twe earhcn atems are ccnnected by a dcuble hcnd, any atcms immediately asscciated with the twc carhcns are: A. at right angles in each ether E- in the same plane I3. in a Hawcrth ccnfiguraticn relatiye tc each cther D. asymmetric 2. Examples cf ncnccyalent hcnds include cf all the fellcwing except A. hydrcgen bend _ El. icnic band C. hydrcphchic bend El. cxygen-hydrcgen he nd 3- Fcr an enzyme reacticn that shcws typical Michaelis—Menten kinetics, dcuhling the ccncentraticn cf enzyme will a. triple the Hm El. dcuhle the Km :3. halye the Him D. nct alter the Him 4. Which cf the iellewing methcds can separate particles hased cn density? A. centrifugaticn _, El. icn exchange chrcmatcgraphy _ C. EDS pclyacrylamide gel electrcphcresis D. affinity chrcmatcgraphy 5. The ability cf DNA tc denature is impcrtant fer which princess? _ a. DNA synthesis _ EL nucleic acid hybridization experiments _ 13. RNA synthesis El. all cf the abcye E. Which cf the fellcwing is net required fcr hcth DNA replicaticn and area transcriptiun? _ Fl- DNA _ El- primers {3. RNA D. pmteins ?. E. if]. 11. 12. A bacteriat eperen eentains at] ef the tetlewing features except: a. multiple transcriptien start sites _ i3. multiple eistrens _ t3. multiple rieeseme binding sites D. multiple genes eneeding enzymes inyelyed in a related functicn All ef the fellewing statements aheut replieatien erigins are tms except: _ s- a bacterial chremeseme has ene erigin ef rsplicatien _ B. s eukaryetic chremeseme has ene erigin ef replieatien _ C. Fleplieatien erigins are unique DNA segments that eentain multiple shert repeated sequences D. Fiaplicatien erigins usually eentain an fitT-rieh streteh What experimental technique was utilized te shew that Iii-hi.va1 replicated by e eemicensewatiy-e preeess? A. density gradient equilibrium centrifugatien ___ El. fiber euterediegraphy G. electren micrescepy E}. gel eleetrepheneeie _-.—. Which ef the fellewfng actiyities is net needed fer replieatien cf the E. eeif ehremeseme’? _ A. telemerase El. Iigase C. primase D. peiymerase _|_- Whieh ef the fellewing is net a hydrepheeic amine aeid'? __ A. alanine _ Ei. tyreeine _ C. serine D. yaline Menerneric preteins de net eentain a a. primary structure B. sece ndary structure :3. tertiary structure _ D. quaternary structure Name SAQl : Desen'he the types of bendsfintereetiens that held together er stabilise the primary, seeenelery, tertiary, and QUHIEI'I'IEIFF structures ef preteins. SAGE: A deuhle—strended pieee ef DNA ennteining the sequenee GCATGGCCACTACCG hes a higher Tm then ene eenteining the sequenee GAATGGTAACAACTG. Describe the preperties ef DNA that make this true. Name 1'} Cenaitlcr the mixture ef the fellewing menemerlc prctcina: {2t} Peinta} a} C} The preteine are first separated according te charge en an leeclcctric fecuaing gel {ace pt—t gel helcw}- Draw and label herizenta] lines in the pH gel tc indicate the relative pcaiticn cf each cftheae 5 preteina after electrcphcrceia. pH-l pH Gel pH T game After separation rat" the five pretetna hp iacclcctric fecuaing, tlte gel is acakecl in SDS and electrcphcresecl an an 5133 pelyaclylamide gel {aaaumc the above {111 gel is the same as used helcw}. Uh the SDS gel, indicate the final relative peaitiena yea weuld expect tc find the preteine. H4 h1D tap {-1 — hettcm {+} If' yen emplcyecl Gel-Filtratleh chrcmategraph}r te separate these preteina, in what crcler weuld ycu expect them tc elute'?l {I} If yen empleyecl Inn—Exchange Ehrflmfltflgrflphy, uaing haeie heads, which preteina weuld elute eff the celumn‘? ...
View Full Document

Page1 / 4

Sample Midterm - 1 When twe earhcn atems are ccnnected by a...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online