Specialized Bacterial Culture Media - Specialized Bacterial...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Specialized Bacterial Culture Media 1. Minimal Salts Base (use glucose for Burkholderia ) Per Liter NH 4 Cl 1 g KH 2 PO 4 1.09 g MgSO 4 . 7H 2 O 0.2 g Trace Salts Solution* 10 mL Carbon source (glucose, sucrose, mannose, etc.) 0.1% pH to 7.0 and add 15 g agar per liter for solid medium *Trace Salts: Prepared in advance in 0.1 N HCl and added to the salts base before sterilization: 300 mg FeSO 4 . 7H 2 O 180 mg MnCl 2 . 4H 2 O 130 mg Co(NO 3 ) 2 . 6H 2 O 40 mg ZnSO 4 . 7H 2 O 20 mg H 2 MoO 4 1.0 mg CuSO 4 . 5H 2 O 1.0 g CaCl 2 HCl (0.1 N) up to 1000 ml 2. Emtiazi et al.** Medium (use glucose for Burkholderia ) Component Per Liter NH 4 Cl 2.5 g KH 2 PO 4 5.4 g Na 2 HPO 4 4.7 g MnSO 4 0.2 g NaCl 0.1 g Carbon source (glucose, sucrose, mannose, etc.) 0.1% pH to 7.0 and add 15 g agar per liter for solid medium Reference: Emtiazi, G., Shakarami, H., Nahvi, I. and Mirdamadian, S. H. Utilization of petroleum hydrocarbons by Pseudomonas sp. and transformed Escherichia coli . African Journal of Biotechnology, Vol. 4, No. 2, February 2005, pp. 172-176.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
2 Media for Frankia and Various Streptomyces -like Bacteria Baker and O’Keefe’s Defined Propionate (Glucose) Medium Component Per liter Na Propionate 1.2 g KH 2 PO 4 1.0 g MgSO 4 .
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern