Standard Media Used In Nodulation Studies

Standard Media Used In Nodulation Studies - Standard Media...

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Standard Media Used In Nodulation Studies 1. Jensen’s Agar Slants Recipe for one liter: CaHPO 4 1.0 g K 2 HPO 4 (or 0.26 g K 2 HPO 4 . 3H 2 O) 0.2 g MgSO 4 . 7H 2 O 0.2 g NaCl 0.2 g FeCl 3 0.1 g Hoagland's micronutrients (see next page) 5 mL pH to 6.8; add 12 g agar There will be a precipitate. Autoclave ca. 1 min. or put into a microwave oven (for small amounts only, no aluminum foil cap!!!) to melt the agar. Stir continuously while dispensing 10 mL of molten medium into 18 x 150 mm test tubes (make certain that the tubes are really clean). Cap the tubes when finished, and then autoclave for the normal time. Remove the tubes from the autoclave when completed and put them on their sides to form slants.
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2. Quarter-Strength Hoagland’s Medium Make up 1 M stock solutions of all salts. Autoclave everything. FeEDTA is a solution of an iron complex of ethylenediaminetetraacetic acid. Each mL of the stock solution contains 5 mg of Fe. Each mL of the FeCl 3 stock solution also contains 5 mg of Fe. To make FeEDTA, dissolve 7.45 g Na 2 EDTA in 1 L H 2 O, then add 5.57 g FeSO 4 . 7H 2 O. Autoclave stock.
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