ASSIGNMENT 1 BIOMAGNIFICATION, HABER’S RULE, HAZARD ASSESSMENT AND PROBIT ANALYSIS ENVR/BOTN/ZOOL/AGRI 2180 and 2190 Introductory Toxicology This assignment is worth 15% of your final grade and is due at the startof class on Tuesday, February 20th, 2007. The total marks for this assignment are 50. It is advised to do any calculations etc., and even the entire answer, in rough before filling in the answer sheet. Marks will be deducted for poor grammar, syntax and spelling as well as answers that are unclear or disorganized. Use onlythe space provided to respond and attached require graphs to the answer sheet with a staple. The graphs must be done using graphing software, such as Excel, SigmaPlot or other available software. Name: __________________________________ Student Number: __________________________ HABER’S RULE 1) Using Haber’s Rule on the data set in Table 1, plot the relationship and report the equation between time and the concentration required to cause a specific effect as a linear function. (6 marks) Remember to log transform the values!2) You estimate the concentration of carbamate required to kill 50% of the Daphnia magnaafter 48 hours of exposure to be _2.08 or 2.05 or 2.1 ug/L_.(1 mark.) Table 1:Acute toxicity data for Daphnia magnaexposed to the carbamate insecticide carbofuran. Time LC50(μg/L) 12 8.4 24 4.2 72 1.4 96 0.98 168 0.61 BIOMAGNIFICATION 11) You are concerned about possible biomagnification of a perfluorinated surfactant, in this case, PFOS (perfluorooctance sulfonate), in arctic ecosystems. PFOS was one of the key ingredients in Scotchgard, formerly produced by 3M. To see if there are any concerns, you have sampled various zooplankton, fish, birds, marine and terrestrial mammals for PFOS in their tissue (see Table 2). The next step is to examine the trends in the data and plot the relationship between concentration versus trophic level. Before you do this, you need to determine the trophic level of each organism and normalize the tissue 1
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