JR midterm ANSWER KEY

JR midterm ANSWER KEY - Page 1 of 10 MBIO 3410 Molecular...

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Page 1 of 10 MBIO 3410 Molecular Biology Midterm (40%) October 23, 2007 Name: Student Number : Time for completion: 70 minutes Maximum marks available: x Provide answers to the following questions in the space provided (note the marks assigned to each question): feel free to provide diagrams and answers in point form; but when diagrams are provided they need to be properly labeled (for DNA or RNA molecules indicate 5' and 3' ends). SECTION 1: MULTIPLE CHOICE QUESTIONS 1. T4 DNA ligase… a. Requires ATP b. Joins double-stranded DNA fragments with an adjacent 3’-phosphate and 5’-OH c. Requires NADH d. Joins single stranded DNA e. two of the above 2. Which of the following virus(es) copy their RNA genome directly to RNA via RNA replication without a DNA intermediate? a. HIV b. Coronavirus c. Hepatitis C d. Retroviruses e. λ bacteriophage f. all of the above g. none of the above h. a and b only i. b and c only j. a and c only 3. In what region of the interphase chromosome does transcription take place? a. the telomere b. the centromere c. euchromatin d. heterchromatin 4 . In prokaryotes, the lagging strand primers are removed by… a. 3’ to 5’ exonuclease b. RNA polymerse c. DNA polymerase II d. DNA polymerase I E. two of the above
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Page 2 of 10 5. Which has the highest absorption per unit mass at a wavelength of 260 nm? a. Double stranded DNA b. Mononucleotides c. RNA d. Single stranded DNA e. Protein SECTION 2: SHORT-ANSWER QUESTIONS Provide answers to the following questions in the space provided 6. During nucleic acid polymerization nucleotides are added to the extending polymer. a. What is the full name of the bond that is formed to link the extending polymer? 3’,5’-phosphodiester bond b. What enzyme accomplishes this reaction? DNA polymerase c. As an additional nucleotide is added to the extending polymer what small molecule is released as a byproduct of nucleic acid polymerization? Pyrophosphate d. What are the three components that make up a nucleotide (DNA)? Phosphate group Nitrogenous base Deoxyribose 7. What is the T m for the following complimentary oligo? 5’-TACAGCAACAGCAGAAGCCGCTCGT-3’ 3’-ATGCACAAGATCCTGCTCGCCGAAG-5’ T m = 2(11) + 4(14) T m = 78 o C 8. a. What enzymes alter the level of supercoiling in DNA? b. What is the name of the enzyme–to-DNA linkage that enables this process to occur? Topoisomerase Phosphotyrosine linkage
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Page 3 of 10 9. Nucleosomes function to condense DNA in eukaryotic cells during mitosis. a. In this process what are the two roles of histone H1? Name two amino acids involved in the interaction with DNA. Stabilize DNA Organize DNA between nucleosomes Lysine and Arginine 10. The following diagram represents an origin of replication in prokaryotic DNA. What are the names of the proteins represented by ‘A’ and ‘B’? 11
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JR midterm ANSWER KEY - Page 1 of 10 MBIO 3410 Molecular...

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