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Unformatted text preview: Name : _____________ ________ ID # : _____________________ Signature : _____________________ Chem & Biochem 153A-2 Summer 2005 Midterm II (100 pt, 50 min) 1 1) Short answers. a) (5 pt) For each of the following types of covalent modification of proteins/enzymes, indicate an amino acid that may function as the site of modification for that process. (list only one amino acid per modification process; you may use the same amino for different processes). a) Phosphorylation b) Adenylation c) Uridylation d) Methylation e) ADP-ribosylation b) (6 pt) E. coli cultures normally grow optimally at about 37 o C. Given our discussion on maintenance of membrane fluidity, what would the lipid composition of an E. coli cell be like if grown at 25 o C? Compare the fatty acid composition of the two conditions relative to chain lengths and unsaturations. c) (6 pt) What is the effect on the binding of O 2 to hemoglobin (increased or decreased affinity) if the pH of the environment increases. Explain fully in terms of molecular interaction, T/R state transformation, and O 2 binding affinity....
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- Summer '06