classes_summer07_153A-2ID18_Midterm_II_S05_Key - Name ID #...

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Name : _________ Key _ ________ ID # : _____________________ Signature : _____________________ Summer 2005 Midterm II (100 pt, 50 min) 1
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1) Short answers. a) (5 pt) For each of the following types of covalent modification of proteins/enzymes, indicate an amino acid that usually functions as the site of modification. (list only one amino acid per modification process; you may use the same amino for different processes). a) Phosphorylation Tyr, Ser, Thr, or His b) Adenylation Tyr c) Uridylation Tyr d) Methylation Glu e) ADP-ribosylation Arg, Gln, or Cys b) (6 pt) E. coli cultures normally grow optimally at about 37 o C. Given our discussion on maintenance of membrane fluidity, what would the lipid composition of an E. coli cell be like if grown at 25 o C? Compare the fatty acid composition of the two conditions relative to chain lengths and unsaturations. At 25 C, membrane fluidity is lower compare to 37 C. Therefore cells will most likely compensate by decreasing fatty acid chain length and increasing unsaturations in fatty acids. Both conditions tend to increase membrane fluidity. c) (6 pt) What is the effect on the binding of O 2 to hemoglobin (increased or decreased affinity) if the pH of the environment increases. Explain fully in terms of molecular interaction, T/R state transformation, and
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This note was uploaded on 07/09/2008 for the course BIO CHEM 153A taught by Professor Staff during the Summer '06 term at UCLA.

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classes_summer07_153A-2ID18_Midterm_II_S05_Key - Name ID #...

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