Sordaria Paper - Sordaria Summary Megan Lawless Biology 104...

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Sordaria Summary Megan Lawless Biology 104 Kathryn Bushley 3-5-2008
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Introduction- Sordaria Fimacola is an ascomycete fungus that produces eight ascospores in each ascus, which are contained in perithecia. Because of the thin, long shape of the ascus, the positioning of the ascospores reflects the arrangement of homologous chromosome pairs during metaphase I of meiosis. Using this knowledge, we were able determine map locations for the tan spore and gray spore genes with respect to the centromere and to conclude whether genes are linked or unlinked. Methods- Three crosses were done in this study. During the first week, we obtained data on the gray spore black spore cross (gt + x g + t + ) and the tan spore black spore cross (g + t x g + t + ). For the tan spore, mutant strain and black spore, wild type strain cross, we obtained a petri dish containing the fungi with mature perithecia. We squashed the perithecia on a microscope slide in order to see the asci. We were concerned only with the perithecia that contain interstrain asci,
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