Lecture_08 - Lecture 8 Bacterial Motility and Chemotaxis...

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1 Lecture 8 Lecture 8 BBOM 11e Sec. 4,10,4.14, 4.16, 5.1-5.2 Exopolymers, Pili (Fimbriae) Bacterial Motility and Chemotaxis Bacterial Nutrition-- Quiz #1 Lectures 1-6 Bacterial Flagella Bacterial Flagella ( A rchaea have them too!!) ( A rchaea have them too!!) Function(s) Long, thin (d~20 nm), helical filaments Peritrichous flagella Polar flagella Motility and Taxis attached to PMF-driven basal motor in cell membrane Molecular motor in cell membrane driven by PMF Structure
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2 Switch Proteins Bacterial Flagellar Motor Structure Bacterial Flagellar Motor Structure The Filament- Hook-basal motor complex embedded in the Gram-negative envelope of E. coli L-ring L-ring P-ring P-ring MS-ring MS-ring H+ Flagellin Subunits Hook Proteins Flagellar filament PMF H+ X 1000/rotation Motor Proteins C-ring C-ring Motile Behavior of E.coli Motile Behavior of E.coli Read: BBOM 11e Sec. 4.14 Two Types of Behavior Depending on the direction of rotation of flagella CW= Tumble CCW = Run Tracking Light Microscope, Dark Field, DIC or Fluorescence Microscopy
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3 Net effect: Cells spend more time running in higher concentrations of attractant. They move up the attractant gradient.
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Lecture_08 - Lecture 8 Bacterial Motility and Chemotaxis...

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