PO_Lecture_04 - Lecture 4 Finish The Big Tree of Life...

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Lecture 4 Properties of Viruses BBOM 11e Sec. 2.1-2.2 Sec. 9.1-9.3, 9.5-9.7 Definition and General Properties of Microorganisms Finish The Big Tree of Life
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SSU rRNA A good molecular chronometer 1. 2. 3. 4. Universal Distribution All cells have ribosomes; therefore, all have SSU rRNA. Functional Constancy Participates in protein synthesis in all cells Alignability Homologous regions with universally conserved sequences Appropriate rate of change for Large and small E d - Many constant and variable regions A “Goldilocks” Chronometer Molecule Just the right length, not too long, not too short! 5.
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Small Subunit (SSU) rRNA 16S rRNA of E.coli SSU rRNA Reference Molecule (for Molecular Phylogeny) 30S Small Subunit 16S rRNA ~1540 Nucleotides Escherichia coli 70S Ribosome Start Finish
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Measuring Evolutionary Distances with SSU rRNA Sequences Count number of differences at each base position 3/12 Use distance computer algorithm to generate a phylogenetic tree showing best fit of corrected E d. Compute corrected E D Determine sequence of SSU rRNA see BBOM 11e Sec. 11.6 Another method called parsimony can be used to generate approximately the same tree.
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ALL PROKARYOTES The Big Tree of Life 5 KINGDOMS??
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PO_Lecture_04 - Lecture 4 Finish The Big Tree of Life...

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