Spring 07 MT 2 Key

Spring 07 MT 2 Key - LS4 Midterm II INSTR: L. Johnson...

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1 LS4 Midterm II INSTR: L. Johnson Monday, May 14, 2007 NAME:_________________________________ (last) (first) I.D. #___________________________________ Signature _______________________________ INSTRUCTIONS 1. This exam is 6 pages long. Problem Value Score Before starting make sure you have all the pages. 1 10 2. Be sure to write clearly. Only 2 12 legible answers will be graded. 3 1 2 4 8 5 2 0 6 18 7 20 Total 100
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2 NAME:_________________________________ ID# _______________________ (last) (first) (10 pts) 1. Consider the cross: Hfr pro+ gal+ arg+ str r cys- met- x F - pro- gal- arg- str s cys+ met+ where the genetic map is shown below (the Å indicates the Origin of transfer in the Hfr strain). Galactose (gal) is a sugar, streptomycin (str) is an antibiotic and the rest of the markers are nutritional. a) What media would you use for the master plate if you wanted to map the distances between each of the markers by interrupted mating? MM+arginine+cysteine (5 pts) b) What media would you use if you wanted to determine the relative distances between str, arg and gal? MM + streptomycin+proline+arginine (+cysteine optional) (5 pts) (12 pts) 2. An Hfr str s arg+ lys+ bio+ is mated to a F - str r arg- lys- bio- , and the mating is stopped by agitation after 45 minutes. The mating mixture is then serially diluted and 0.1 ml are plated as indicated below. Media supplements 0 10 -2 10 -4 10 -6 Strep, arginine, lysine, biotin Conf Conf Conf 30 Strep, arginine, lysine Conf 600 6 0 Strep, arginine, biotin Conf 400 4 0 Strep, lysine, biotin 30 0 0 0 a) What is the frequency of transfer of arg+ ? 30/(30 x 10 6 ) = 1x10 -6 (4 pts) b) What is the frequency of transfer of lys+ ? (4 x 10
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This note was uploaded on 07/13/2008 for the course LS 4 taught by Professor Ribaya during the Spring '08 term at UCLA.

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Spring 07 MT 2 Key - LS4 Midterm II INSTR: L. Johnson...

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