Working with E. coli

Working with E. coli - \ilorking with E coli Intnoduction...

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\ilorking with E coli Intnoduction Wor*ing with bacteria is very oonunor in Molecular Biology. Bactcia are Gasy to grow 'and are easily transformable with foreign DNA Bacteda can provide an excellem syst m to select and amplify genes of imere*. The common species ofbacteria usd is.E @rr. .Plasmids can be easilytransformed into.E, coli Plasmids are uzually doubte-strande4 closed circular DNA molocules that can be manipulated to carry DNA of interest firom prokar5rctes, animals orplants. lvlany plasmids rely on hos machineryto provide tbe enzymes to replicate but lhey behave as aocessory gencic nnits and are inheritod independently of the bacterid c,hrornosomes For your first experimed you will be given three types of bacterid cuhrc labeled: A B and C. Determine the idemity of each bacterid culnre: l. E olithrt does nd oontain a plasmid. 2. E coli carrying a plasmid (pBSK) with a resistance gene to ampicillin. This plasmid also comains the or s meril of the gere for p-galactosidase. the
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This note was uploaded on 07/13/2008 for the course MCDB 104 taught by Professor Lee during the Summer '08 term at UCLA.

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Working with E. coli - \ilorking with E coli Intnoduction...

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