quizanswers

quizanswers - TA Quizzes-Answers 1) 1 1 2 3 4 2 + 3 + 4 + +...

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TA Quizzes—Answers 1) 1 2 3 4 1 - + - + 2 - + + 3 - + 4 - How many genes are identified by this analysis? 3 From row 1, we see that mutation 1 fails to complement mutation 3, so they are in the same gene. Mutations 2 and 4 complement mutation 1, so they are not in the same gene as mutation 1. 2 and 4 are not necessarily in the same gene, we must keep looking at the chart to figure that out. All we know so far is that mutations 2 and 4 are not in the same gene as mutation 1 and 3. From row 2, we see that mutation 2 complements mutation 4, so they must also be in different genes. Which mutants belong to the same complementation groups? 1 complementation group (mutations in same gene) – mutations 1 and 3 mutation 2 is in a different gene than 4 and (1 and 3) mutation 4 is in a different gene than 2 and (1 and 3) 2) 1) You have two mutant organisms expressing the same phenotype and want to know whether the mutations are in the same gene. What type of test can you do to determine this? _______ complementation test 2) What do you need to determine before you can do the test listed for question 1? Determine if the mutation is recessive. If recessive, then proceed to complementation test. 3) If you cross the two mutant organisms from question 1 and get a wild type phenotype, are the mutations in the same gene or different genes? Different genes 4) Are the two mutations from question 3 in the same complementation group? No 5) Can point mutations spontaneously revert to wild type? yes Can deletions spontaneously revert to wild type? no 3) (1) What is the purpose of a complementation test? To determine if two mutations with the same phenotype occur in the same gene or in different genes (2) What is one criterion the mutations in question must fit in order for a complementation test to be a valid experiment?
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Mutations must be recessive (3) Why are complementation tests more straightforward in most eukaryotes than in bacteria or viruses? Most eukaryotes are diploid or are sometimes diploid as a part of their life cycle (Also accept easier to score phenotypes) (4) Is this an example of complementation or of failure to complement? Complementation 4) You are a geneticist working with T4 phage and have discovered an interesting mutation that slows the rate at which the phage is able to produce plaques on a lawn of E. coli. You have been able to isolate 2 strains of phage that show this phenyotype. You wish to know if the strains have mutations in the same or different genes, so you perform a complementation study. Upon co-infection you see the mutant phenotype, i.e. slow growing plaques. Based on this study alone, would you conclude that the mutations are in the same gene or in two different genes? Since the strains do not complement each other the mutations are likely in the same
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This note was uploaded on 07/14/2008 for the course LS 4 taught by Professor Ribaya during the Fall '08 term at UCLA.

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quizanswers - TA Quizzes-Answers 1) 1 1 2 3 4 2 + 3 + 4 + +...

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