Midterm_II_Key

Midterm_II_Key - Short Questions Question 1(5 points The...

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1 Short Questions Question 1 (5 points) The rII locus consists of two cistrons, A and B. You identified 6 mutations in the rII locus. You know for sure that rII-1 is in cistron A. Map these mutations based on the following information. +: plaques on bacterial lawn; - : no plaques on bacterial lawn. Specify in which gene these mutations are located. (Use parenthesis, if map positions are ambiguous) Complementation test Recombination test 1 2 3 4 5 6 1 2 3 4 5 6 1 - + - + - - 1 - + - + + + 2 - + - + - 2 - + + + - 3 - + - - 3 - + + - 4 - + - 4 - + + 5 - - 5 - + 6 - 6 - A B 5 1 2 4 3 6 Question 2. (8 points) All the following mutants require compound K for growth on minimal medium. The compounds A to E in the biosynthesis pathway to K are known but their order in the pathway is not known. Each compound is tested for its ability to support the growth of each mutant (1 – 5) in minimum medium. The results are shown below. A + means growth and a 0 means no growth. Draw the biochemical pathway. Compound tested A B C D E K Mutant 1 0 + 0 + + + 2 + + 0 + + + 3 0 0 0 0 0 + 4 + 0 + + + + 5 0 + 0 + 0 + C A 4 B E 2 D K 1 5 3
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2 Long Questions Question 1 (12 points) You have isolated 6 different mutations in the rII locus of bacteriophage T4 and have constructed the following double mutants. These mutants map to a non-essential region of the rII locus. The mutations are either frameshift mutations or nonsense mutations. Mutation 1 is a (+) frameshift mutation. Shown below are the plaque phenotypes the phage make when plated on a lawn of E. coli B or E. coli B (Su-). E. coli B(Su-) is a mutant strain of E. coli B which suppresses nonsense mutations. Plaque phenotype when grown on: Mutant B B (Su-) wild type small small 1 large large 2 large large 3 large small 4 large large 5 large large 6 large large 1, 2 small small 1, 3 large large 1, 4 small small 1, 5 large large 1, 6 large large 2, 4 large large 3, 5 large large (a)
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