Lecture 4 - Lecture#4 James K Miyasaka...

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Lecture #4 James K. Miyasaka February 13, 2008 [email protected] Factors Affecting RBC Antigen-Antibody Reactions Speaker: Gregory Garraty, PhD, FRCPath Scienctific Director American Red Cross Blood Services Southern California Region Methods for Detecting Antigen-Antibody Reactions: (methods for blood transfusion) Precipitation : older ways look at it as lines of identity that merge into one product. Complement fixation : test for sifilys, uses sheep cells as antibody. Hemolysis : use routinely complement Agglutination : used every day. Inhibition: sample mixed in saline solution and A, B, and O will be in the saline. Labeled antibodies : routinely applied, binds to RBC and labels the antibody. o Flourochromes : (immunofluorescence) o Isotopes(RIA) : use radio isotopes. o Enzymes(EIA) : uses a particular one, it will change the blood color. o Ferritin/gold(EM) : electron microscopy, look for black dots. Chemiluminescence : tagged antibodies give you use laminators. Solid phase RBC indicator : uses for platelets, sold in IGG bonded plate kits. Humagglutination has two stages. Stage 1: Red blood cells moves and causes collisions where antibodies will get on RBC. o Sensitization: antibodies goes onto RBC, bonds to antibody binding site. Stage 2: glutination o Aggulatination: is the clumping of cells together. Important Factors Influencing Binding of Antibody to RBC’s Collision: RBC continuously moving, could collide. The negative charges causes a repel. Affinity(goodness of fit): add antibody to antigen it will reach equilibrium. You can graph it Concentration vs. Time.
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